Electrochemical and Mass Spectrometric Measurement of Enhanced Intravesicular Catecholamine Content and Exocytotic Frequency at Subanaesthetic Ketamine Doses

The dopaminergic system has been suggested to serve as a functional target for ketamine, especially with its addictive and antidepressant effects. In this work, intracellular vesicle impact electrochemical cytometry single cell amperometry were applied model line show that the intravesicular contents number of exocytotic events increased fraction release decreased after treatment by subanesthetic ketamine (1 μM 15 min). addition, time-of-flight secondary ion mass spectrometry was used cellular lipids change following acute administration relative control cells an proportion low-curvature high-curvature lipids. We propose modulates function dose-dependent pharmacologic action dominantly interacting different proteins changing lipid composition. It might be helpful better understand relationship drug abuse, well anesthetic